R E C I P E S

V. DNA Purification and Automated Sequencing

3 M Sodium Acetate (NaOAc, pH 5.2)

Makes 100 ml. Store at room temperature (indefinitely).

  1. Dissolve 24.6 g sodium acetate (MW 82.03 g/mol) in 70 ml of deionized or distilled water.
  2. Adjust the pH to 5.2 by adding glacial acetic acid; monitor with a pH meter.
  3. Add water to bring the total volume of solution to 100 ml.
M I T O C H O N D R I A L  C O N T R O L  R E G I O N

DNA Learning Center, Cold Spring Harbor Laboratory
Noncommercial, educational use only.
Kits available from Carolina Biological Supply Company.